The web-server predMultiLoc-Gneg used to predict the subcellular localization of protein. we propose an efficient multi-label predictors, predMultiLoc-Gneg, to predict the subcellular locations of multi-label gram-negative bacterial proteins. Experimental results show that our method is very promising and can be a useful tool for the prediction of multi-label subcellular location of protein . The predMultiLoc-Gneg has achieved higher success rates in comparison with the existing predictors in this area. For convenience of the experimental scientists, we have established a user-friendly web server and a step by step guide have been provided about how to use this web server. It provides as easier way to obtain the desired results without knowing the mathematical details. We have projected that the predMultiLoc-Gneg will become a very useful and higher throughput tool to deal with both single- and multi-label subcellular locations prediction of multi-label gram-negative bacterial proteins.